TRPγ, a Drosophila TRP–Related Subunit, Forms a Regulated Cation Channel with TRPL

نویسندگان

  • X.-Z.Shawn Xu
  • Fred Chien
  • Alice Butler
  • Larry Salkoff
  • Craig Montell
چکیده

TRP and TRPL are two light-sensitive cation channel subunits required for the Drosophila photoresponse; however, our understanding of the identities, subunit composition, and function of the light-responsive channels is incomplete. To explain the residual photoresponse that remains in the trp mutant, a third TRP-related subunit has previously been proposed to function with TRPL. Here, we identify such a subunit, TRPgamma. We show that TRPgamma is highly enriched in photoreceptor cells and preferentially heteromultimerizes with TRPL in vitro and in vivo. The N-terminal domain of TRPgamma dominantly suppressed the TRPL-dependent photoresponse, indicating that TRPgamma-TRPL heteromultimers contribute to the photoresponse. While TRPL and TRPgamma homomultimers are constitutively active, we demonstrate that TRPL-TRPgamma heteromultimers form a regulated phospholipase C- (PLC-) stimulated channel.

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عنوان ژورنال:
  • Neuron

دوره 26  شماره 

صفحات  -

تاریخ انتشار 2000